Second story mode:
     Clear  all 100 floors in story mode to access a second story mode
with 30 floors.

Unlock Bonus characters:
     Clear  all 100 floors in story mode to unlock the following bonus
characters:

Tsunade 
Sasuke 
Jiraiya 
Orochimaru 
Kabuto 
Shizune 

     Clear  all  30  floors  in 2nd story mode to unlock the following
bonus characters:

Kisame 
3rd Hokage
Itachi